Cell Biolabs Inc His-Tag Protein ELISA Kit, 96 assays

Supplier:  Cell Biolabs Inc AKR130

Often used for affinity purfication of recombinant proteins in various expression systems, the 6xHis-tag motif provides a powerful purification tool while minimizing any effect on the protein’s functionality and bioactivity, making it a popular tag for recombinant protein production and isolation. However, the accurate quantitation of His-tagged protein in unpurified samples remains difficult. Cell Biolabs’ His-Tag Protein ELISA Kit is a competitive enzyme immunoassay developed for rapid quantitation of His-tagged protein samples (C- or N-terminal). The quantity of His-tag in a protein sample is determined by comparing its absorbance with that of a His-tag protein standard curve (provided). The kit has detection sensitivity range of 4 µg/mL to 1 ng/mL (10 kDa His-tag protein standard), or 400 nM - 100 pM 6xHis-tag residues. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and His-tag samples.

Catalog No. NC0409249