Thermo Scientific™ HisPur™ Cobalt Purification Kit, 1 mL

Description

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.

Additional Information and Online Ordering

HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co²⁺)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni²⁺chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.

Highlights:

• High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions

• Low metal leaching – no metal contamination in eluted histidine-tagged protein sample

• Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives

• Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates

• Cost effective – resin can be reused several times or discarded after single use

• Superior – performs better than other commercially available IMAC Resins

Available Formats:

• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
• Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin

Includes:

Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

Specifications

• Capacity (Metric): 5 columns
• Description: HisPur Cobalt Spin Purification Kit, 1mL
• Content And Storage: Sufficient For: Purifying ∼10 mg of His-tagged protein per column
  • HisPur Cobalt Spin Columns, 1 mL, 5 columns
  • Equilibration/Wash Buffer, 100 mL
  • Elution Buffer, 50 mL
  
  Store at 4°C.
• Product Line: HisPur™
• Quantity: 5 columns
• Target: His-Tagged Protein
• Type: Protein Purification System
• Stationary Phase: Metal Chelate, Divalent Cobalt
• Sufficient For: 10 mg of His-Tagged Protein per Column
• Format: Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.