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Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.

Manufacturer:  Thermo Scientific™ 25228

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Catalog No. PI25228


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Description

Description

Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.


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HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps.

Highlights:

  • Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator

  • High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate

  • High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins

  • Compatible – maintains function after exposure to a wide variety of chemicals and pH values

  • Cost effective – resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Large scale FPLC purification of polyhistidine tagged proteins

Specifications

Specifications

Agarose Resin, Affinity
HisPur™
Bottle(s)
10mL
Store at 4°C
His-tagged Proteins
Metal Chelate, Divalent Cobalt
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