Thermo Scientific HisPur Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Manufacturer: Thermo Scientific 25228
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt.
Robust highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
High purity specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible maintains function after exposure to a wide variety of chemicals and pH values
Cost effective resin is stable through multiple cycles of cleaning and reuse
Large scale FPLC purification of polyhistidine tagged proteins
|Agarose Resin, Affinity|
|Store at 4°C|
|Metal Chelate, Divalent Cobalt|