Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads.
High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast – protocol is completed in 1 hour
Scalable – process microliter to milliliter sample volumes
Versatile – purify proteins using native or denaturing conditions
Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)
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