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  6. Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Catalog No. PI25217
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Catalog No. Quantity
PI25217 1 L
PI25214 10 mL
PI25215 50 mL
PI25216 250 mL
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Catalog No. PI25217

Supplier: Thermo Scientific™ 25217

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Description

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Additional Information and Online Ordering

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur
Quantity 1 L
Target His-Tagged Protein
Type Agarose
Stationary Phase Divalent Nickel, Metal Chelate
Format Bottle
Column Type Affinity, Agarose Resin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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