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Innovative Research Inc Hmn Acrosin Kit
Supplier: Innovative Research Inc IHUACRKT
Human Acrosin ELISA Kit - This kit is for the quantitative determination of Human Acrosin in biofluid samples, such as sperm. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . ACR present in samples reacts with an anti-Human ACR capture antibody coated and dried on a microtiter plate. After washing steps, an anti-ACR detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active ACR in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human ACR. Detection Target Human Acrosin Reactivity Human Range 0.156-10ng/mL Sensitivity 0.062ng/mL Storage Conditions Store all components at 4C For RUO.
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