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Innovative Research Inc Hmn Rcptr Asst Prt Rcmb Stbl M
Supplier: Innovative Research Inc IHURAPRSM500UG
Human Receptor-Associated Protein Recombinant Stable Mutant 500ug - Recombinant Stable Mutant is recombinantly produced in E. coli and purified by affinity chromatography. This is a Frozen liquid buffered in 0.02M HEPES; 0.15M NaCl; 0.001M CaCl2; pH 7.5 and a purity of >95% as determined by SDS-PAGE. Six mutations were made in the D3 domain (Y260 & T297 changed to cystines, generating a novel disulfide bond between helices 2 & 3; H257, H259, H268, & H290 changed to phenylalines). This is a stable mutuant that is resistent to both pH and heat-induced denaturation. This mutant will not unfold at pH 5.5, has a melting temperature of 73C, and is more resistant to trypsin- and chymotrypsin-mediated proteolysis. This product can be used as a control in applications like ELISA and Western Blot. It can be used for a wide range of in vitro applications, including blocking, cell binding, cell differentiation, cell signaling, protein-protein interactions, receptor binding, and more
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