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Innovative Research Inc Hmn Ttl PAI1 Kit
Supplier: Innovative Research Inc IHUPAI1KTT
Human Total PAI-1 ELISA Kit - This human plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is for the quantitative determination of total PAI-1 in human plasma in citrate, EDTA or heparin, serum, cell culture media, tissue lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Human PAI-1 will bind to the monoclonal capture antibody coated on the microtiter plate. Free, latent, and complexed PAI-1 will bind to the plate. After appropriate washing steps, polyclonal anti-human PAI-1 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1. Color development is proportional to the concentration of PAI-1.
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