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Apexbio Technology LLC Hoechst 33342/PI Double Staining Kit 500T
Supplier: Apexbio Technology LLC K2237500
Hoechst 33342 PI Double Staining Kit is a fluorescence-based assay kit designed to distinguish apoptosis and necrosis in cell populations The kit utilizes two fluorescent probes Hoechst 33342 and propidium iodide (PI) to assess plasma membrane integrity and nuclear morphology Hoechst 33342 penetrates live cells and binds to DNA producing blue fluorescence with intensity increasing upon apoptotic chromatin condensation PI is impermeable to intact cell membranes and selectively labels necrotic cells with red fluorescence Compared to single-stain apoptosis assays this dual-stain approach enables simultaneous detection of viable apoptotic and necrotic cells Based on these properties Hoechst 33342 PI Double Staining Kit is applicable in pharmacological evaluation and cytotoxicity studies
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