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INTACT GENOMICS INC Hot Start Taq 2x Master Mix 500 Reactions
Supplier: INTACT GENOMICS INC 3296
Hot start Taq DNA Polymerase 2x master mix is a ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with Intact Genomics 5X Magic Enhancer for efficient amplification of GC rich templates to 84%.Applications Routine PCR and RT-PCR Primer extension Colony PCR Genotyping Efficient for amplifying high GC content template DNA with Magic Enhancer.
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