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Mayflower Bioscience HOTFIREPol EvaGreen qPCR ROX

Supplier: Mayflower Bioscience 081400001

HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
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