Abnova HPV Screening CISH Probe
HPV Screening CISH Probe is designed for the qualitative detection of human papillomavirus (HPV) type 6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82 DNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).
Manufacturer: Abnova CG0026
DescriptionThe probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit, which provides necessary reagents for specimen pretreatment and post-hybridization processing.
The staining pattern in the nucleus can be observed as discrete and dot-shaped signals in case of integrated HPV, or as a strong and homogeneous nuclear staining in case of episomal HPV. A cytoplasmic staining is observed when RNA sequences of HPV are detected. Visualization of signals should be performed using a set of objectives ranging from an at least 200-fold to 630-fold magnification. The presence of the episomal staining pattern is usually detected clearly by an objective with 200-fold magnification, whereas the detection of the integrated HPV pattern requires a greater magnification, preferably 630-fold. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei and nuclei with weak signal intensity.
|The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (37°C) and mix briefly before use.|