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New England Biolabs, Inc. HiScribe T7 mRNA Kit with CleanCap Reagent AG
Supplier: New England Biolabs, Inc. E2080S
The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail mRNAs can be transcribed with a 5-m7G Cap-1 structure that is polyadenylated translationally competent and able to evade the cellular innate immune response. The kit is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs with a total kit yield of 1.8 mg of mRNA. Cap-1 mRNA synthesized from this kit is suitable for many applications including transfections microinjections in vitro translation preclinical mRNA therapeutic mRNA studies as well as RNA structure and function analysis.
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