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BioVendor, LLC Recombinant protein 0.1 mg, 1/ea
Supplier: BioVendor, LLC RD172263100
Recombinant protein Description Total 111 AA. MW: 12.0 kDa (calculated). UniProtKB acc.no. P61604. N-Terminal His-tag (10 extra AA)Amino Acid SequenceMKHHHHHHASAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVDSourceE. coliPurity: >95% Endotoxin< 0.1 EU/?gFormulationFiltered (0,4 ?m) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4ReconstitutionAdd deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.ApplicationsWestern blotting, ELISAQuality Control TestBCA to determine quantity of the protein.SDS PAGE to determine purity of the protein.LAL to determine quantity of endotoxin.
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