Invitrogen™ Human HSP70 ELISA Kit

Catalog No.	Quantity
50-112-5289	96 Tests

Catalog No. 50-112-5289 Supplier Invitrogen™ Supplier No. BMS2087

Description

ELISA

The Human Heat Shock Protein 70 (Hu HSP70) ELISA quantitates Hu HSP70 in human serum, plasma, cell lysates or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP70. Principle of the method The Human HSP70 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Specifications

• Accession Number: P34932
• Assay Range: 0.156-10.0 ng/mL
• Assay Sensitivity: 0.052 ng/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human HSP70
  Biotin-Conjugate anti-human HSP70 monoclonal antibody
  Streptavidin-HRP
  Human HSP70 Standard lyophilized, 20 ng/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 70kDa; AI317151; APG2; APG-2; epididymis secretory sperm binding protein Li 5a; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock 70-related protein APG-2; Heat shock protein; heat shock protein 4; heat shock protein Apg-2; heat shock protein family A (Hsp70) member 4; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; Hsp110; hsp70; hsp70 RY; Hsp70RY; Hspa4; HSPH2; Irp94; ischemia responsive 94 kDa protein; mKIAA4025; RY70kDa; AI317151; APG2; APG-2; epididymis secretory sperm binding protein Li 5a; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock 70-related protein APG-2; Heat shock protein; heat shock protein 4; heat shock protein Apg-2; heat shock protein family A (Hsp70) member 4; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; Hsp110; hsp70; hsp70 RY; Hsp70RY; Hspa4; HSPH2; Irp94; ischemia responsive 94 kDa protein; mKIAA4025; RY; APG-2; HEL-S-5a; HS24/P52; HSPH2; RY; hsp70; hsp70RY
• Gene ID (Entrez): 3308
• Gene Symbol: HSPA4
• Interassay CV: 4.5%
• Intraassay CV: 5.3%
• Kit Contents: Pre-coated 96 well plates, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: HSP70RY, Heat shock 70-related protein APG-2
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2-8°C
• Synonym: Heat Shock Protein 70
• Target: HSPA4
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr