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Invitrogen™ Human HSPB1 (HSP27) ELISA Kit

Catalog No. EHHSPB1X10
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EHHSPB1X10 10 x 96 Tests
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ELISA

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
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Specifications

Accession Number P04792
Assay Range 0.205 to 50 ng/mL
Assay Sensitivity 150 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3315
Gene Symbol HSPB1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target HSP27
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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