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Lonza Walkersville Hu CD4/CD45RA Naive T-Cells
Supplier: Lonza Walkersville 2W202
Na ve CD4 T cells are negatively selected using a two step isolation process CD4 T Cells are first negatively selected from mononucleated cells obtained from leukapharesed blood using an indirect immunomagnetic CD4 labeling system CD45RO microbeads are then used to deplete the CD45RO population leaving a highly enriched CD4 CD45RA Na ve T Cell population Cells are cryopreserved in Lonza Cryo Media in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using hemacytometer (trypan blue method) Cells are collected from healthy donors following IRB protocols
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