Monocytes are found in the circulating peripheral blood where they make up 10-20% of the total mononuclear cell population Monocytes are characterized by their phenotypic expression of CD14 and thus are commonly called CD14 monocytes They play an important role in host defense as circulating monocytes and differentiation into tissue macrophages and can differentiate into dendritic cells with potent antigen-presenting capability Cells You Can Count on to PerformLonza Human Peripheral Blood CD14 Monocytes are isolated from peripheral blood of screened healthy untreated (non-mobilized peripheral blood) donors Briefly mononuclear cells (MNCs) are isolated from apheresis derived peripheral blood The MNC population is then further purified via positive immunomagnetic selection directed against CD14 Cryopreserved CD14 Monocytes are guaranteed to express CD14 and will contain either 10 20 or 40 million viable cells per ampoule depending upon the product ordered