This recombinant Human Fc gamma RIIIA (CD16a, V176) protein is an intermediate-affinity, polypeptide-anchored transmembrane glycoprotein expressed in human HEK293 cells. It serves as a receptor for the Fc portion of IgG antibodies and plays a critical role in immunological processes, including phagocytosis, secretion of inflammatory mediators, and antibody-dependent cytotoxicity. The protein contains a C-terminal polyhistidine tag and has a calculated molecular weight of 23.7 kDa, migrating at 40–60 kDa in SDS-PAGE due to glycosylation.
- Expressed in human HEK293 cells for appropriate post-translational modifications
- Purity >95% as determined by SDS-PAGE and SEC-MALS
- Lyophilized in PBS, pH 7.4, with trehalose protectant
- Endotoxin level <1.0 EU per μg by LAL method
- Validated for binding applications using SPR and BLI