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ACROBiosystems Recombinant Protein;Human PD-L1 / B7-H1 Protein, Fc Tag (HPLC-verified);HEK293;100UG;PD1,PDL1,PD-L1,CD274,B7-H1,B7H1,B7-H,B7H,PDCD1L1,PDCD1LG1
Supplier: ACROBiosystems PD1H5258100UG
Recombinant Protein;Human PD-L1 / B7-H1 Protein, Fc Tag (HPLC-verified);100UG;Human PD-L1, Fc Tag (HPLC-verified) (PD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).;This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.4 kDa. The protein migrates as 60-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human PD-1, Strep Tag (Cat. No. PD1-H5284) at 2 μg/mL (100 μL/well) can bind Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) with a linear range of 4-63 ng/mL (QC tested).;Immobilized Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) at 5 μg/mL (100 μL/well) can bind Anti-PD-L1 MAb with a linear range of 0.2-2 ng/mL (Routinely tested).;Flow Cytometry assay shows that recombinant Human PD-L1, Fc Tag (HPLC-verified) (Cat. No. PD1-H5258) can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 10 μg/mL (Routinely
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