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ACROBiosystems Recombinant Protein;Human SIRP alpha / CD172a Protein, mouse IgG1 Fc Tag (HPLC-verified);HEK293;100UG;SIA,SIRP alpha,SHPS1,SIRPA,CD172A,BIT,SHPS-1,MFR,MYD-1,MYD1,PTPNS1,SIRPalpha,p84,SIRP-alpha,CD172
Supplier: ACROBiosystems SIAH52A8100UG
Recombinant Protein;Human SIRP alpha / CD172a Protein, mouse IgG1 Fc Tag (HPLC-verified);100UG;Human SIRP alpha, Mouse Fc Tag (SIA-H52A8) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).;This protein carries a mouse IgG1 Fc tag at the C-terminus, and has a calculated MW of 63.5 kDa. The predicted N-terminus is Glu 31. The reducing (R) protein migrates as 70-105 kDa in SDS-PAGE due to glycosylation.;Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 2 μg/mL can bind Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) with a linear range of 8-250 ng/mL (QC tested).;FACS assay shows that recombinant Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 μg/ml (Routinely tested).;FACS analysis shows that the binding of Human SIRP alpha, mouse IgG1 Fc Tag (HPLC-verified) (Cat. No. SIA-H52A8) to Jurkat express
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