Gibco™ Human 4-1BB Ligand (TNFSF9) Recombinant Protein

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -80°C to -20°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.

Specifications

• Accession Number: P41273
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 5 mM tris with 75 mM NaCl and no preservative; pH 8
• Gene ID (Entrez): 8744
• Molecular Weight (g/mol): 19.5 kDa
• Name: Human 4-1 BB Ligand (TNFSF9)
• Purification Method: Sequential Chromatography
• Quantity: 20 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: 4 1 BB Ligand; 4 1 BBL; 41 BB Ligand; 4-1 BB ligand; 41 BBL; 4-1 BBL; 4-1 BB-L; AI848817; CD 137 Ligand; CD 137 L; CD137 Ligand; CD137L; Cd157l; CDw137L; costimulatory molecule; homolog of mouse 4-1 BB-L; Ly63l; receptor 4-1 BB ligand; TNFRSF9; Tnfsf9; TNLG5A; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5 A; tumor necrosis factor ligand superfamily member 9; tumor necrosis factor superfamily member 9
• Common Name: 4-1 BBL (CD137L)
• Biological Activity: ED50 = 5 - 10 ng/mL; determined by the dose-dependent stimulation of IL-8 production by human PBMCs.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human 4-1 BB Ligand recombinant protein contains 185 amino acids
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Immunology, Cardiovascular Research, Signal Transduction, Apoptosis
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC