Invitrogen™ Human A-GHRL ELISA Kit

Catalog No.	Quantity
EEL181	96 Tests

Catalog No. EEL181 Supplier Invitrogen™ Supplier No. EEL181

Description

ELISA

The Human Acylated Ghrelin (A GHRL) ELISA quantitates A GHRL in serum, plasma and other biological fluids. Principle of the method The Human Acylated Ghrelin (A GHRL) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Acylated ghrelin is a hormone primarily produced by the stomach and released into the bloodstream. It plays a significant role in regulating appetite and energy balance. Functionally, acylated ghrelin acts as an orexigenic hormone, meaning it stimulates appetite and promotes food intake. It interacts with specific receptors in the brain, particularly the growth hormone secretagogue receptor 1a (GHSR1a), to increase hunger signals and enhance food-seeking behaviors. Acylated ghrelin also influences energy homeostasis by increasing fat storage and reducing energy expenditure. Structurally, acylated ghrelin is a peptide hormone consisting of 28 amino acids. It undergoes a post-translational modification process called acylation, where a fatty acid (usually octanoic acid) is attached to the third amino acid residue (serine) in the peptide chain. This acylation is crucial for the hormone's biological activity and stability. Localization-wise, acylated ghrelin is primarily produced by the X/A-like cells in the gastric mucosa. After secretion into the bloodstream, it acts on various target tissues, including the hypothalamus, pituitary gland, and adipose tissue. The hypothalamus is particularly important for regulating appetite and energy balance, as it receives and integrates signals from acylated ghrelin.

Specifications

• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: A-GHRLA-GHRL;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Tissue Homogenate 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Acylated Ghrelin
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min