Human a1BG Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of a1BG in Serum plasma and other biological fluids It provides a detection range of 0 32-20 ng/mL with a sensitivity of 0 154 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1BG Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1BG Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human a1BG biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color