Gibco™ Human Activin A Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Activin A in sterile PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA. Storage: Store this lyophilized preparation at <-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at <-20°C. Avoid repeated freeze/thaw cycles.

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.

Specifications

• Accession Number: P08476, Q04998
• For Use With (Application): Control, ELISA, Functional Assay, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 16323, 3624
• Molecular Weight (g/mol): 25 kDa
• Name: Human Activin A
• Purification Method: Gel-Purified
• Quantity: 10 μg
• Endotoxin Concentration: <1 EU/mL
• Gene Alias: activin A; activin beta-A chain; BMP7; BMP-7; Bone morphogenetic protein 7; EDF; Eptotermin alfa; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FRP; FSH-releasing protein; INHB; Inhba; inhibin B subunit; inhibin beta A chain; inhibin beta A protein; inhibin beta A subunit; inhibin beta-A; inhibin beta-A-subunit; inhibin beta-subunit; inhibin subunit beta A; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); Inhibin, beta-1; OP1; OP-1; Osteogenic protein 1; unnamed protein product
• Common Name: Activin A
• Biological Activity: ED50 = 0.5 - 5 ng/mL; determined by the dose-dependent proliferation of MPC-11 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Content And Storage: -20°C
• Classification: Carrier-Free
• Activity: 0.5 ng/ml
• Bioassay: Inhibition of Proliferation of MPC11 cells
• Endotoxin Level: <1 EU/ml
• Shipping Condition: Dry Ice
• Expression System: HEK293 cells
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Activins
• Research Category: Neurobiology, Stem Cell Research, Differentiation
• Product Line: Gibco™
• Purity or Quality Grade: >95% by SDS-PAGE

Safety and Handling

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