Invitrogen™ Human Adiponectin ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
Biotin-Conjugate anti-human Adiponectin monoclonal antibody
Streptavidin-HRP
Human Adiponectin Standard lyophilized, 30ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

ELISA

The Human Adiponectin ELISA quantitates Hu Adiponectin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Adiponectin. Principle of the method The Human Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

Specifications

• Accession Number: Q15848
• Assay Range: 0.23 to 15 ng/mL
• Assay Sensitivity: 0.012 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 9370
• Gene Symbol: ADIPOQ
• Interassay CV: 3.1%
• Intraassay CV: 4.2
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2°C to 8°C
• Synonym: ACDC, apM1, GBP-28, ADIPOQ
• Target: Adiponectin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins