Invitrogen™ Human Adiponectin ProQuantum Immunoassay Kit

Description

ELISA

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

Specifications

• Accession Number: Q15848
• Assay Range: 0.76 to 1,500,000 pg/mL
• Assay Sensitivity: <0.7 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Immunoassay Kit Format: Proximity-based Immunoassay
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 9370
• Incubation Time: 1 hr.
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: Frozen at -20°C
• Target: Adiponectin
• Test Time: 30 mins
• Total Assay Time: 2 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov