Invitrogen™ Human ADP-Ribosyl Cyclase 2 ELISA Kit

Catalog No.	Quantity
EH15RBX5	5 x 96 Tests
EH15RB	96 Tests

Catalog No. EH15RBX5 Supplier Invitrogen™ Supplier No. EH15RBX5

Description

ELISA

Human ADP-Ribosyl Cyclase 2 quantitates human ADP-Ribosyl Cyclase 2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ADP-Ribosyl Cyclase 2.

CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST1), is a pleiotropic ectoenzyme and glycosylphosphatidylinositol-anchored molecule that functions both as an enzyme and a receptor. Similar to CD38, CD157 is involved in the metabolism of NAD+, which may play a role in regulating intracellular Ca2+ levels. As an enzyme, CD157 is also referred to as ADP-ribosyl cyclase 2 and DAMP-1, and it facilitates pre-B cell growth. As a receptor, CD157 is thought to initiate a signal transduction cascade upon binding to its putative ligand, leading to the phosphorylation of cytoplasmic proteins, including focal adhesion kinase (FAK). While the mechanism and functional significance of CD157-initiated signal transduction are not fully characterized, its role in cellular signaling is evident. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis, suggesting a link to polyclonal B cell abnormalities observed in the disease. This overexpression in the stromal cell population may contribute to the pathophysiology of rheumatoid arthritis, highlighting the importance of CD157/BST1 in both normal immune function and disease states.

Specifications

• Accession Number: Q10588
• Assay Range: 0.06-12.50 ng/mL
• Assay Sensitivity: 0.06 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 114/A10; A530073F09; ADP-ribosyl cyclase 2; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; antigen BP3; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; Bp3; Bp-3; BP-3 alloantigen; Bst1; BST-1; Bsta1; cADPr hydrolase 2; CD157; CD157 antigen; Cyclic ADP-ribose hydrolase 2; leukocyte antigen 65; Ly65; Ly-65; NAD(+) nucleosidase; NAD+ nucleosidase114/A10; A530073F09; ADP-ribosyl cyclase 2; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2; antigen BP3; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; Bp3; Bp-3; BP-3 alloantigen; Bst1; BST-1; Bsta1; cADPr hydrolase 2; CD157; CD157 antigen; Cyclic ADP-ribose hydrolase 2; leukocyte antigen 65; Ly65; Ly-65; NAD(+) nucleosidase; NAD+ nucleosidase;
• Gene ID (Entrez): 683
• Gene Symbol: Bst1
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: CD157 (BST1)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov