Invitrogen™ Human ALKP ELISA Kit

Catalog No.	Quantity
EH19RBX5	5 x 96 Tests
EH19RB	96 Tests
EH19RBX10	10 x 96 Tests

Catalog No. EH19RBX5 Supplier Invitrogen™ Supplier No. EH19RBX5

Description

ELISA

Human ALKP quantitates human ALKP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ALKP.

Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.

Specifications

• Accession Number: P05186
• Assay Range: 2-500 mIU/mL
• Assay Sensitivity: 2 mIU/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, intestinal; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPI; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; IAP; intestinal alkaline phosphatase; intestinal-type alkaline phosphatase; Kasahara isozyme; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific alkaline phosphatase precursor; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-APAkp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, intestinal; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPI; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; IAP; intestinal alkaline phosphatase; intestinal-type alkaline phosphatase; Kasahara isozyme; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific alkaline phosphatase precursor; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP;
• Gene ID (Entrez): 249
• Gene Symbol: ALPL
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: ALPL
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min