Invitrogen™ Human alpha-1 PDX Recombinant Protein

Catalog No.	Quantity
RP070	2.5 mg

Catalog No. RP070 Supplier Invitrogen™ Supplier No. RP070

Description

Recombinant Protein

RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20 μM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 μM alpha1-PDX should be used. Purity: >60% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays. Molecular weight is approximately 46.7 kDa.

Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors.

Specifications

• Concentration: 2.5 mg/mL
• For Use With (Application): Control, Inhibition Assay
• Formulation: PBS with no preservative; pH 7.2
• Molecular Weight (g/mol): 46.7 kDa
• Name: Human alpha-1 PDX
• pH Range: 7.2
• Quantity: 2.5 mg
• Source: E. coli
• Regulatory Status: RUO
• Gene Alias: alpha1 PDX; Pyruvate Dehydrogenase Complex Component-X
• Common Name: alpha-1 PDX
• Biological Activity: For furin inhibition assays, cells should be treated with 8.0 - 20 μM human alpha-1 PDX recombinant protein for 1 hour. For in vitro assays, 0.1 - 2.0 μM human alpha-1 PDX recombinant protein should be used.
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Content And Storage: 4°C, do not freeze
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >80%

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov