Human alpha-synuclein pre-formed fibrils (tag-free) are recombinant human alpha-synuclein (Met 1-Ala 140) assembled into amyloid fibrils and supplied as a trehalose-stabilized preparation for use in in vitro seeding assays, cell-based models, and in vivo studies. Material is expressed in E. coli and validated for fibril formation and activity by biochemical and imaging assays.
- Contains amino acids Met 1-Ala 140.
- Expressed in E. coli.
- Pre-formed fibril format ready for sonication and PFF assays.
- Purity greater than 90% by SDS-PAGE.
- Migrates at 15-16 kDa on SDS-PAGE.
- Supplied in PBS, pH 7.4, 0.2 μm filtered, with trehalose protectant.
- Available in 100 μg, 500 μg, and 1 mg sizes.
- Store at -70 to -80 °C; avoid repeated freeze-thaw and storage at 4 °C.
- Validated by Thioflavin T assay and transmission electron microscopy.