Invitrogen™ Human Aminopeptidase N/ANPEP ELISA Kit

Description

ELISA

Human Aminopeptidase N/ANPEP quantitates human Aminopeptidase N/ANPEP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Aminopeptidase N/ANPEP.

CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells, and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus.

Specifications

• Accession Number: alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase; aminopeptidase M; Aminopeptidase N; aminopeptidase N/CD13; ANPEP; Apm; AP-M; Apn; AP-N; bAPN; Cd13; cluster of differentiation antigen 13 (CD13); gp150; h AP-N; hAPN; kidney aminopeptidase M; kidney Zn peptidase; KZP; Lap1; Lap-1; leucine arylaminopeptidase 1; membrane alanine aminopeptidase; membrane alanyl aminopeptidase; membrane protein p161; Microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; P150; PEPN; rAPN; Unknown (protein for MGC:126949)
• Assay Range: 0.041 to 10 ng/mL
• Assay Sensitivity: 0.041 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 290
• Gene Symbol: ANPEP
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD13
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

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