Invitrogen™ Human Angiotensin 2 ELISA Kit

Catalog No.	Quantity
EEL024	96 Tests

Catalog No. EEL024 Supplier Invitrogen™ Supplier No. EEL024

Description

ELISA

The Human Angiotensin II (Ang-II) ELISA quantitates Ang-II in serum, plasma and other biological fluids Principle of the method: The Competitive Angiotensin II (Ang-II) ELISA research-use-only kit is designed to quantitatively measure Ang-II in Humans. An Ang-II standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human Ang-II is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human Ang-II in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Specifications

• Accession Number: P01019
• Assay Range: 31.25-2000 pg/mL
• Assay Sensitivity: 18.75 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Serpin
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AGT; alpha-1 antiproteinase, antitrypsin; Ang I; Ang II; Ang III; Ang IV; Angiotensin 1-10; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 1-9; Angiotensin 2-8; Angiotensin 3-8; Angiotensin I; angiotensin II; Angiotensin III; Angiotensin IV; Angiotensin-1; Angiotensin-2; Angiotensin-3; Angiotensin-4; Angiotensinogen; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); ANHU; Des-Asp[1]-angiotensin II; FLJ92595; FLJ97926; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; serpin A8; serpin peptidase inhibitor, clade A, member 8; SERPINA8AGT; alpha-1 antiproteinase, antitrypsin; Ang I; Ang II; Ang III; Ang IV; Angiotensin 1-10; Angiotensin 1-4; Angiotensin 1-5; Angiotensin 1-7; Angiotensin 1-8; Angiotensin 1-9; Angiotensin 2-8; Angiotensin 3-8; Angiotensin I; angiotensin II; Angiotensin III; Angiotensin IV; Angiotensin-1; Angiotensin-2; Angiotensin-3; Angiotensin-4; Angiotensinogen; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); ANHU; Des-Asp[1]-angiotensin II; FLJ92595; FLJ97926; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; serpin A8; serpin peptidase inhibitor, clade A, member 8; SERPINA8;
• Gene ID (Entrez): 183
• Gene Symbol: AGT
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Angiotensin II
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min