The Human SerpinA8 / Angiotensinogen / AGT ELISA Pair Set is for the quantitative determination of Human SerpinA8 / Angiotensinogen / AGT.This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for SerpinA8 / Angiotensinogen / AGT coated on a 96-well plate. Standards and samples are added to the wells, and any SerpinA8 / Angiotensinogen / AGT present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-SerpinA8 / Angiotensinogen / AGT polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of SerpinA8 / Angiotensinogen / AGT present in the sample. To end the enzyme reaction, the stop solution is added a