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MP Biomedicals™ Human APO L1, Apolipoprotein L1 ELISA Kit

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Description

Quantitative detection of human APOL1/Apolipoprotein L1 in samples of cell culture supernatant, saliva, urine, serum, plasma, cell lysates, tissue homogenates, and other biological fluids.

Human apolipoprotein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles. The human ApoL protein family is involved in lipid transport and metabolism. APOL1 is also involved in the host's innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect humans from infection. Genetic variants in the APOL1 gene, which are found in African ancestry with high frequency, are associated with chronic kidney diseases, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy.

  • In this ELISA kit, an APOL1/Apolipoprotein L1 specific antibody has been pre-coated onto 96-well plates and blocked
  • Standards or test samples are added to the wells and subsequently an APOL1/Apolipoprotein L1 specific biotinylated detection antibody is added and followed by washing with wash buffer
  • Afterwards, Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer
  • TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction
  • TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes to yellow after adding an acidic stop solution
  • The density of yellow coloration is directly proportional to the amount of APOL1/Apolipoprotein L1 captured in the plate
TRUSTED_SUSTAINABILITY

Specifications

Assay Sandwich Assay
Conjugate APO L1
Cross Reactivity None
Immunoassay Kit Format 96-well Plate
Label Type Biotin
Product Line MP Biomedicals
Product Type ELISA Kit
Protein Family Apolipoprotein
Sample Type Blood, Plasma, Serum, Tissue Homogenates
Specificity No Cross-reactivity to Other Antigens
Substrate TMB
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase)
Sufficient For 96-well Plate
Target Species Human
Quantity 96 Preps
Regulatory Status RUO
Storage Requirements 2°C to 8°C
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

Unless specified otherwise, MP Biomedical™'s products are for research or further manufacturing use only, not for direct human use.

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