Abnova™ Human APOB native Protein from human plasma

Description

Used for SDS-PAGE

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq]

Specifications

• For Use With (Application): SDS-PAGE
• Formulation: Lyophilized from 1 ml of 10mM sodium deoxycholate, 50mM NaCl, 5 0mM Na
• Gene ID (Entrez): 338
• Name: APOB (Human) Native Protein
• Preparation Method: Purified from low density lipoproteins (LDL) isolated by density gradient ultracentrifugation (1.03 - 1.05 g/mL density range) and by gel filtration chromatography in 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na₂CO₃, pH 10.1.
• Purification Method: Density gradient ultracentrifugation and gel filtration chromatography
• Quantity: 500 μg
• Storage Requirements: Store at -20°C or lower on dry atmosphere. After reconstitution, store at 2-8°C no longer than 4 weeks. Reconstitution: Dissolve lyophilized Apo B in 100 mL to 1 mL of distilled or deionized water. If dissolving in solvent we recommend using 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na₂CO₃, pH 10 to keep the sodium deoxycholate at an acceptable level instead of water.
• Regulatory Status: RUO
• Gene Alias: FLDB
• Common Name: APOB
• Gene Symbol: APOB
• Species: Human
• Protein Tag: None
• Expression System: Purified from low density lipoproteins (LDL) isolated by density gradient ultracentrifugation (1.03 - 1.05 g/mL density range) and by gel filtration chromatography in 10 mM sodium deoxycholate, 50 mM NaCl, 50 mM Na₂CO₃, pH 10.1.
• Form: Lyophilized
• Purity or Quality Grade: ≥95% by SDS-PAGE