Invitrogen™ Human Argonaute 3 (aa 133-171) Control Fragment Recombinant Protein

Catalog No.	Quantity
RP100945	100 μL

Catalog No. RP100945 Supplier Invitrogen™ Supplier No. RP100945

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61716. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

AGO3 (protein argonaute-3) is required to RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. AGO3 is proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.

Specifications

• Accession Number: Q9H9G7
• Concentration: 5.90 mg/mL
• For Use With (Application): Neutralization, Control
• Formulation: PBS, 1M urea with no preservative; pH 7.4
• Gene ID (Entrez): 192669
• Name: Human Argonaute 3 (aa 133-171) Control Fragment
• pH Range: 7.4
• Purification Method: Purified
• Quantity: 100 μL
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: AGO3; argonaute 3; argonaute 3, RISC catalytic component; argonaute RISC catalytic component 3; argonaute RISC catalytic subunit 3; argonaute3; AW048688; C130014L07Rik; eIF2C 3; eIF-2C 3; EIF2C1; EIF2C3; Eukaryotic translation initiation factor 2C 3; eukaryotic translation initiation factor 2C, 1; eukaryotic translation initiation factor 2C, 3; hAgo3; mAgo3; Piwi/Argonaute family protein meIF2C3; Protein argonaute-3
• Common Name: Argonaute 3
• Gene Symbol: AGO3
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: WHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLP
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining