Biotinylated Human B7-H7 (HHLA2) is a recombinant Fc-tagged extracellular domain produced in human 293 (HEK293) cells and supplied lyophilized with trehalose. It is enzymatically biotinylated for site-specific binding assays and has been MALS-verified for quality, making it suitable for bioactivity ELISA applications targeting CD28H.
- Recombinant extracellular domain of human B7-H7 (HHLA2) expressed in HEK293 cells.
- Human IgG1 Fc tag for dimerization and detection.
- Site-specific enzymatic biotinylation at a single lysine residue.
- Purity >95% by SDS-PAGE and >90% by SEC-MALS.
- Calculated MW 65.1 kDa; apparent 70-90 kDa on reducing SDS-PAGE due to glycosylation.
- Lyophilized formulation with trehalose; formulated in Tris/glycine/arginine/NaCl buffer.
- Validated for bioactivity ELISA binding to CD28H with QC-tested linear range in the low ng/mL.