Human BAFF/CD257 Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of BAFF/CD257 in serum plasma tissue homogenates and other biological fluids It provides a detection range of 78 13-5000 pg/mL with a sensitivity of 29 pg/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257 Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257 Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human BAFF/CD257 biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color