Gibco™ Human BDNF Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute BDNF in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing carrier protein, such as 0.1% BSA and store in working aliquots from -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for up to 1 month, but should be kept at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Accession Number: P23560
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 627
• Molecular Weight (g/mol): 27 kDa
• Name: Human BDNF
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Common Name: BDNF
• Biological Activity: ED50 = 0.5 - 1.0 μg/mL; determined by the dose-dependent proliferation of rat C6 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human BDNF recombinant protein is a homodimer containing two 120 amino acid subunits linked by hydrophobic interaction
• Content And Storage: -20°C
• Classification: Carrier-Free
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Neurotrophic Factors
• Research Category: Neurobiology, Stem Cell Research, Differentiation
• Product Line: Gibco™
• Purity or Quality Grade: ≥98% by SDS-PAGE and HPLC

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov