Invitrogen™ Human Beta Endorphin ELISA Kit

Catalog No.	Quantity
EEL031	96 Tests

Catalog No. EEL031 Supplier Invitrogen™ Supplier No. EEL031

Description

ELISA

The Human Beta-Endorphin (beta-EP) ELISA quantitates beta-EP in serum, plasma and other biological fluids Principle of the method: The Competitive Beta-Endorphin (beta-EP) ELISA research-use-only kit is designed to quantitatively measure beta-EP in Humans. A beta-EP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human beta-EP is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human beta-EP in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Beta endorphin is an opioid neuropeptide that is produced by neurons of the pituitary gland and released into the circulatory system, or produced by the hypothalamus, and released into the brain and spinal cord through nerve fibers. The precursor polypeptide for beta endorphin is pro-opiomelanocortin.

Specifications

• Accession Number: P01189
• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: POMC
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ACTH; Adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; alpha-MSH; Beta-endorphin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; CLIP; Corticotropin; Corticotropin-like intermediary peptide; corticotropin-lipotropin; Gamma-LPH; gamma-MSH; Lipotropin beta; lipotropin gamma; LPH; Melanocyte-stimulating hormone alpha; Melanocyte-stimulating hormone beta; Melanotropin alpha; melanotropin beta; Melanotropin gamma; met-enkephalin; MSH; NPP; opiomelanocortin prepropeptide; OTTHUMP00000119991; OTTHUMP00000200964; POC; POMC; Potential peptide; Precursor of MSH; pro-ACTH-endorphin; proopiomelanocortin; pro-opiomelanocortin; proopiomelanocortin preproproteinACTH; Adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; alpha-MSH; Beta-endorphin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; CLIP; Corticotropin; Corticotropin-like intermediary peptide; corticotropin-lipotropin; Gamma-LPH; gamma-MSH; Lipotropin beta; lipotropin gamma; LPH; Melanocyte-stimulating hormone alpha; Melanocyte-stimulating hormone beta; Melanotropin alpha; melanotropin beta; Melanotropin gamma; met-enkephalin; MSH; NPP; opiomelanocortin prepropeptide; OTTHUMP00000119991; OTTHUMP00000200964; POC; POMC; Potential peptide; Precursor of MSH; pro-ACTH-endorphin; proopiomelanocortin; pro-opiomelanocortin; proopiomelanocortin preproprotein;
• Gene ID (Entrez): 5443
• Gene Symbol: POMC
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Beta Endorphin
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min