Human BRAK Microsample ELISA Kit is a Sandwich-based immunoassay designed for the quantitative measurement of BRAK in Serum plasma tissue homogenates cell lysates cell culture supernates and other biological fluids It provides a detection range of 0 16-10 ng/mL with a sensitivity of 0 05 ng/mL The assay can be completed in approximately 3 5h The procedure follows a The test principle applied in this kit is Sandwich enzyme immunoassay The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRAK Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRAK Next Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated After TMB substrate solution is added only those wells that contain Human BRAK biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color