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Invitrogen™ Human Brevican ELISA Kit

Catalog No. EH52RBX5
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EH52RBX5 5 x 96 Tests
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Catalog No. EH52RBX5 Supplier Invitrogen™ Supplier No. EH52RBX5
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Description

ELISA

Human Brevican quantitates human Brevican in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Brevican.

Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number ALPBRE; Bcan; BEHAB; Brain-enriched hyaluronan-binding protein; brevican; brevican (brain specific proteoglycan in the aggrecan family); brevican core protein; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; CSPG7; UNQ2525/PRO6018
Assay Range 0.041 to 10 ng/mL
Assay Sensitivity 0.041 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 63827
Gene Symbol Bcan
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Brevican
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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