Invitrogen™ Human C1q ELISA Kit

Catalog No.	Quantity
50-112-5330	96 Tests
BMS2099TWO	2 x 96 Tests
BMS2099TEN	10 x 96 Tests

Catalog No. 50-112-5330 Supplier Invitrogen™ Supplier No. BMS2099

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C1q

ELISA

The Human C1q ELISA quantitates Hu C1q in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C1q. Principle of the method The Human C1q solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.

Specifications

• Accession Number: P02745, P02746, P02747
• Assay Range: 0.9-60 ng/mL
• Assay Sensitivity: 0.08 ng/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human C1q
  Biotin-Conjugate anti-human C1q monoclonal antibody
  Streptavidin-HRP
  Human C1q Standard lyophilized,120 ng/mL upon reconstitution
  Sample Diluent
  Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Stop Solution (1M Phosphoric acid)
  Adhesive Films
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AI255395; AI385742; C1q; C1qa; C1qb; C1qc; C1Q-C; C1QG; Ciqc; complement C1q A chain; complement C1q B chain; complement C1q C chain; complement C1q chain A; complement C1q chain B; complement C1q subcomponent subunit A; complement C1q subcomponent subunit A-like protein; Complement C1q subcomponent subunit B; Complement C1q subcomponent subunit C; complement component 1, q subcomponent, A chain; complement component 1, q subcomponent, alpha polypeptide; complement component 1, q subcomponent, B chain; complement component 1, q subcomponent, beta polypeptide; complement component 1, q subcomponent, C chain; complement component 1, q subcomponent, c polypeptide; complement component 1, q subcomponent, gamma polypeptide; complement component C1q, A chain; complement component C1q, B chain; complement subcomponent C1q chain BAI255395; AI385742; C1q; C1qa; C1qb; C1qc; C1Q-C; C1QG; Ciqc; complement C1q A chain; complement C1q B chain; complement C1q C chain; complement C1q chain A; complement C1q chain B; complement C1q subcomponent subunit A; complement C1q subcomponent subunit A-like protein; Complement C1q subcomponent subunit B; Complement C1q subcomponent subunit C; complement component 1, q subcomponent, A chain; complement component 1, q subcomponent, alpha polypeptide; complement component 1, q subcomponent, B chain; complement component 1, q subcomponent, beta polypeptide; complement component 1, q subcomponent, C chain; complement component 1, q subcomponent, c polypeptide; complement component 1, q subcomponent, gamma polypeptide; complement component C1q, A chain; complement component C1q, B chain; complement subcomponent C1q chain B;
• Gene ID (Entrez): 712, 713, 714
• Gene Symbol: C1QA, C1QB, C1qc
• Interassay CV: 4.2%
• Intraassay CV: 4.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
• Storage Requirements: 2-8°C
• Synonym: complement component 1, q subcomponent, A chain el, complement component 1, q subcomponent, alpha poly
• Target: C1q
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 30 min