Invitrogen™ Human C1qTNF5 ELISA Kit

Description

ELISA

Human C1qTNF5 quantitates human C1qTNF5 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human C1qTNF5.

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP5 has been suggested to be involved in age-related macular degeneration.

Specifications

• Accession Number: C1q and tumor necrosis factor related protein 5; C1q TNF-alpha-related protein 5; C1QTNF5; Complement C1q tumor necrosis factor-related protein 5; Ctrp5; LORD; Membrane frizzled-related protein; Membrane-type frizzled-related protein; Mfrp; myonectin; UNQ303/PRO344
• Assay Range: 0.82 to 200 ng/mL
• Assay Sensitivity: 0.82 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 114902
• Gene Symbol: C1QTNF5
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CTRP5
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins