Invitrogen™ Human Complement C3a ELISA Kit

Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human C3a

ELISA

The Human Complement C3a (Hu C3a) ELISA quantitates Hu C3a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C3a. Principle of the method The Human C3a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.

Specifications

• Accession Number: P01024
• Assay Range: 0.3 to 20 ng/mL
• Assay Sensitivity: 0.14 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 718
• Gene Symbol: AHUS5,ARMD9,ASPa,C3b,CPAMD1,HEL-S-62 p,C3
• Interassay CV: 5.9%
• Intraassay CV: 5.9
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: complement component 3, ASP, C3a, C3b, C3, Acylation stimulating protein
• Target: Complement C3a
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 30 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov