Invitrogen™ Human CA13 ELISA Kit

Catalog No.	Quantity
EH59RBX5	5 x 96 Tests
EH59RB	96 Tests
EH59RBX10	10 x 96 Tests

Catalog No. EH59RBX5 Supplier Invitrogen™ Supplier No. EH59RBX5

Description

ELISA

Human CA13 quantitates human CA13 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CA13.

Carbonic anhydrase XII (CA XII), encoded by the CA12 gene, is one of the enzyme isoforms in the carbonic anhydrase family that catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons. CA XII is a transmembrane protein expressed in various tissues, including the kidneys, intestines, and lungs, playing crucial roles in maintaining acid-base balance, pH regulation, and electrolyte transport. Its activity is significant in organs where fluid secretion and absorption are essential processes, impacting functions such as respiration and renal bicarbonate reabsorption. CA XII is involved in physiological processes like cell proliferation and differentiation by modulating the extracellular and intracellular pH, influencing tumor microenvironment dynamics. Aberrant expression of CA XII has been noted in several cancers, including breast and colorectal cancers, where it supports tumor growth and metastasis by maintaining the acid-base homeostasis conducive to cancer cell survival. Due to its surface expression and role in maintaining pH balance, CA XII is a target of interest for therapeutic interventions aiming to disrupt tumor progression and enhance the efficacy of cancer treatments.

Specifications

• Accession Number: Q8N1Q1
• Assay Range: 24-6000 pg/mL
• Assay Sensitivity: 24 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 2310075C21Rik; Ca13; Car13; Carbonate dehydratase XIII; carbonic anhydrase 13; Carbonic anhydrase XIII; CAXIII; CA-XIII; RGD15604532310075C21Rik; Ca13; Car13; Carbonate dehydratase XIII; carbonic anhydrase 13; Carbonic anhydrase XIII; CAXIII; CA-XIII; RGD1560453;
• Gene ID (Entrez): 377677
• Gene Symbol: Ca13
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Carbonic Anhydrase XIII
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov