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Invitrogen™ Human Carboxypeptidase B2/CPB2 ELISA Kit

Catalog No. EH68RBX5
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ELISA

Human Carboxypeptidase B2/CPB2 quantitates human Carboxypeptidase B2/CPB2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Carboxypeptidase B2/CPB2.

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for its gene and its promoter region.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 1110032P04Rik; 4930405E17Rik; AI255929; carboxypeptidase B2; carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); carboxypeptidase B-like protein; carboxypeptidase R; Carboxypeptidase U; Cpb2; CPR; CPU; PCPB; Plasma carboxypeptidase B; Tafi; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
Assay Range 0.082 to 20 ng/mL
Assay Sensitivity 0.082 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1361
Gene Symbol CPB2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Carboxypeptidase B2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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