Molecular Probes™ Human Cardiomyocyte Immunocytochemistry Kit

Catalog No.	Quantity
A25973	50 tests

Catalog No. A25973 Supplier Molecular Probes™ Supplier No. A25973

Description

Human Cardiomyocyte Immunocytochemistry Kit

The Human Cardiomyocyte Immunocytochemistry Kit enables optimal image-based analysis of two key cardiomyocyte markers: NKX2.5 and TNNT2/cTnT. It is the only kit that offers superior imaging for cardiomyocytes in one box, with a complete set of primary and secondary antibodies, a nuclear DNA stain, and all of the pre-made buffers for an optimized staining experiment.

With the Human Cardiomyocyte Immunocytochemistry Kit, you can:

Confirm expression of key cardiomyocyte markers with highly specific antibodies

Get more information per sample by measuring more than one marker at a time

Generate beautiful multiplexed images with minimal effort

Save time and avoid cell loss by eliminating unnecessary wash steps in your staining protocol

Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key Cardiac Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the immunocytochemistry analysis of cardiomyocytes. The antibodies are well suited for the characterization of cardiomyocytes generated from pluripotent stem cells using the Gibco™ PSC Cardiomyocyte Differentiation Kit.

Get More Information Per Sample
The antibodies in the kit have been designed to allow for the specific and simultaneous assessment of NKX2.5 and TNNT2 along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of cardiomyocyte marker expression using commonly available blue, green, and orange/red filter sets.

Eliminate Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard immunocytochemistry protocols, saving time and decreasing the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Human Cardiomyocyte Immunocytochemistry Kit comes with reagents sufficient to stain 50 samples using a 200μL staining volume. Detailed kit contents can be found below.

Specifications

• Conjugate: 2 Color, Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594
• Quantity: 50 tests
• Content And Storage:

  Store at -20°C to 4°C:

  • 10 μL of Mouse Anti-Human TNNT2 (1000X)
  • 10 μL Rabbit Anti-Human NKX2.5 (1000x)
  • 40 μL of Alexa Fluor™ 555 Donkey Anti-Rabbit (250X)
  • 40 μL of Alexa Fluor™ 594 Donkey Anti-Rabbit (250X)
  • 40 μL of Alexa Fluor™ 488 Donkey Anti-Mouse(250X)

  Store at -20°C to RT:

  • 1 vial of NucBlue™ Fixed Cell ReadyProbes™ Reagent (1X)
  • 10 mL of Fixative Solution (1X)
  • 10 mL of Permeabilization Solution S (1X)
  • 20 mL of Blocking Solution (1X)
  • 20 mL of Wash Buffer (10X)
• Product Line: Alexa Fluor
• Product Type: Immunocytochemistry Kit

Frequently Asked Questions (FAQs)

The Human Cardiomyocyte Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Can I use the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis?

We do not recommend using the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Do you offer the individual components of the Human Cardiomyocyte Immunocytochemistry Kit as standalone products?

Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.