Invitrogen™ Human Cathepsin K ELISA Kit

Catalog No.	Quantity
EEL033	96 Tests

Catalog No. EEL033 Supplier Invitrogen™ Supplier No. EEL033

Description

ELISA

The Human Cathepsin K (CTSK) ELISA quantitates Cathepsin K in serum, plasma, and other biological fluids The Human Cathepsin K solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing.

Specifications

• Accession Number: P43235
• Assay Range: 0.16-10 ng/mL
• Assay Sensitivity: 0.1 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Peptidase C1
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: AI323530; Cat K; cathepsin K; Cathepsin O; cathepsin O1; cathepsin O2; Cathepsin X; catK; CTS02; CTSK; CTSO; CTSO1; CTSO2; MGC23107; minisatellite 10q detected by probe MMS10; MMS10-Q; Ms10q; PKND; PYCDAI323530; Cat K; cathepsin K; Cathepsin O; cathepsin O1; cathepsin O2; Cathepsin X; catK; CTS02; CTSK; CTSO; CTSO1; CTSO2; MGC23107; minisatellite 10q detected by probe MMS10; MMS10-Q; Ms10q; PKND; PYCD;
• Gene ID (Entrez): 1513
• Gene Symbol: CTSK
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 100 μL, Serum 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Cathepsin K
• Test Time: 1 hr 20 min
• Total Assay Time: 3 hr 30 min